Kansai University Research: Cell Membrane-inspired Phospholipid Polymers as New Generation of Biomaterials for Medical Devices

However, non-specific binding of proteins to substrates has been much more difficult to overcome because conventional substrates are not compatible with biomolecules, and it is extremely difficult to prevent non-specific attachment of proteins. With the aim of developing biocompatible substrates for diagnostic devices, a team led by Yasuhiko Iwasaki at Kansai University in Japan has patterned substrates using antifouling poly[2-methacryloyloxyethyl phosphorylcholine (MPC) polymers with residues of tyrosine. The researchers demonstrated that a combination of visible light assisted fabrication and the antifouling nature of PC polymers enabled them to control the immobilization of proteins onto specific regions of patterned arrays for applications in immunoassay platforms.

However, non-specific binding of proteins to substrates has been much more difficult to overcome because conventional substrates are not compatible with biomolecules, and it is extremely difficult to prevent non-specific attachment of proteins. With the aim of developing biocompatible substrates for diagnostic devices, a team led by Yasuhiko Iwasaki at Kansai University in Japan has patterned substrates using antifouling poly[2-methacryloyloxyethyl phosphorylcholine (MPC) polymers with residues of tyrosine. The researchers demonstrated that a combination of visible light assisted fabrication and the antifouling nature of PC polymers enabled them to control the immobilization of proteins onto specific regions of patterned arrays for applications in immunoassay platforms.

In their experiments Iwasaki and his colleagues immobilized proteins in so-called zwitterionic polymer networks to prevent denaturation of proteins if they interacted with substrates. Then a series of biochemical procedures were used to define micro-array patterns through TEM mesh masks that were irradiated with white light for 15 s. This process led to microarrays where nonspecific protein immobilization was eliminated on the nonirradiated regions of the surface.

"The immobilization process of proteins was easy, quick, safe, and reliable," says Iwasaki. "This result could serve as a basic design strategy for fabricating biosensors and diagnostic devices."

Video of the visible light irradiation process
http://www.rsc.org/suppdata/d0/cc/d0cc02092c/d0cc02092c2.mp4

Orthopedic medical applications of poly[2-methacryloyloxyethyl phosphorylcholine (MPC)

Iwasaki and his colleagues have also discovered that zwitterionic polymer brushes can be used for producing robust and low friction surfaces with potential medical applications including replacing metals in orthopedic implants.

Specifically, Iwasaki and his group used the photoreactive and water soluble zwitterionic monomer 2[2-(methacryloyloxy)ethyldimethylanmmonium] ethyl benzophenoxy phosphate (MBPP) to improve the stability of zwitterionic polymer brushes.

The researchers selected photosensitive poly(ether ether ketone) (PEEK) as a substrate and immersed it into aqueous solutions of 2-(methacryloyloxy) ethyl phosphorylcholine (MPC) and MBPP. The solution was then irradiated with UV light for 180 min. Optical and XPS measurements showed that this process created low friction-grafting layers of zwitterionic polymers on the PEEK samples.

"The bacterial adhesion was completely suppressed on the surface of PEEK modified with cross-linked PMPC brushes with MBPP," says Iwasaki. "Thus, we conclude that the surface modification of PEEK with MPC and MBPP can provide ideal surface properties for orthopedic devices.

References

General information
Kansai University
3-3-35 Yamate-cho, Suita-shi,
Osaka 564-8680 JAPAN
EMAIL: [email protected] 

Websites
Kansai University
http://www.kansai-u.ac.jp/English/
Kansai University e-bulletin
http://www.kansai-u.ac.jp/Kokusai/e-bulletin/

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